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18F-FACBC PET/MRI in the evaluation of human brain metastases: a case report



Patients with metastatic cancer to the brain have a poor prognosis. In clinical practice, MRI is used to delineate, diagnose and plan treatment of brain metastases. However, MRI alone is limited in detecting micro-metastases, delineating lesions and discriminating progression from pseudo-progression. Combined PET/MRI utilises superior soft tissue images from MRI and metabolic data from PET to evaluate tumour structure and function. The amino acid PET tracer 18F-FACBC has shown promising results in discriminating high- and low-grade gliomas, but there are currently no reports on its use on brain metastases. This is the first study to evaluate the use of 18F-FACBC on brain metastases.

Case presentation

A middle-aged female patient with brain metastases was evaluated using hybrid PET/MRI with 18F-FACBC before and after stereotactic radiotherapy, and at suspicion of recurrence. Static/dynamic PET and contrast-enhanced T1 MRI data were acquired and analysed. This case report includes the analysis of four 18F-FACBC PET/MRI examinations, investigating their utility in evaluating functional and structural metastasis properties.


Analysis showed high tumour-to-background ratios in brain metastases compared to other amino acid PET tracers, including high uptake in a very small cerebellar metastasis, suggesting that 18F-FACBC PET can provide early detection of otherwise overlooked metastases. Further studies to determine a threshold for 18F-FACBC brain tumour boundaries and explore its utility in clinical practice should be performed.


In the field of neuro-oncology, advancing the descriptive breadth and precision of imaging techniques is of paramount importance for describing tumour growth and structure. This information is invaluable in determining a treatment plan, evaluating the efficacy of treatment and detecting tumour recurrence.

Estimations show that 10–40% of patients with extracranial malignant tumours develop brain metastases (Helseth et al. 2003). The average survival time for these patients is less than 6 months (Stelzer 2013; Liu et al. 2019). Furthermore, the incidence of brain metastases is increasing. This is likely a consequence of the prolonged survival, provided by improved systemic therapies of primary tumours and the increased use of imaging techniques such as magnetic resonance imaging (MRI) (Liu et al. 2019; Nayak et al. 2012).

Structural MRI is used as a standard procedure to delineate, diagnose and plan treatment for brain metastases. MRI provides excellent soft tissue contrast, does not involve exposure to radiation and is an especially versatile imaging technique. However, MRI has some limitations, such as micro-metastasis detection, lesion boundary delineation and discrimination between progression and pseudo-progression after treatment (Bogsrud et al. 2019). Dynamic and static data acquired from amino acid (AA) positron emission tomography (PET) could provide the opportunity for a more detailed analysis of brain tumour extent and malignancy (Karlberg et al. 2017; Karlberg et al. 2019). Additionally, AA PET has been shown to detect malignant glioma progression as well as treatment efficacy earlier than MRI (Galldiks et al. 2013; Unterrainer et al. 2016). However, treatment planning using either PET or MRI can underestimate brain metastasis margins (Gempt et al. 2015). These MRI limitations might be reduced or even resolved with the utilisation of PET/MRI (Bogsrud et al. 2019; Galldiks et al. 2017). The combination of PET and MRI is a promising, non-invasive and relatively novel method. It can improve the detection of brain tumours, in addition to advancing precision and navigation during surgical and radiotherapy treatment (Karlberg et al. 2017; Karlberg et al. 2019). Hence, combined PET/MRI data can provide physicians with a better overview in the diagnosis of gliomas and brain metastases and influence clinical decision-making.

Some AA tracers 3,4dihydroxy-6-[18F]-fluoro-L-phenylalanine (18F-FDOPA), [11C]-methyl-methionine (11C-MET) and [18F]-fluoro-ethyltyrosin (18F-FET) are now recommended by international guidelines as a complement to MRI in the evaluation of gliomas (Albert et al. 2016). In contrast to 18F-2-fluoro-2-deoxy-D-glucose (18F-FDG), AA PET tracers show low uptake in healthy brain tissue and increased uptake in tumour cells, resulting in high tumour-to-background ratios (TBRs)—possibly due to an increased expression of AA transporters in tumour cells (Galldiks et al. 2017; Papin-Michault et al. 2016). A few studies also show promising results using 11C-MET and 18F-FET in patients with brain metastases, by reflecting biologic tumour behaviour, delineating tumour margins and differentiating progression from pseudo-progression after treatment (Gempt et al. 2015; Grosu et al. 2011; Unterrainer et al. 2017; Akhoundova et al. 2020).

Anti-1-amino-3-[18F]fluorocyclobutane-1-carboxylic acid (18F-FACBC) is another AA PET tracer with even higher TBRs than other AA PET tracers, which could aid the differentiation between high-grade and low-grade gliomas (Bogsrud et al. 2019; Karlberg et al. 2017; Karlberg et al. 2019). Furthermore, 18F-FACBC can reveal satellite tumours not detected with MRI in high-grade gliomas (Bogsrud et al. 2019). However, no studies have been performed using 18F-FACBC in patients with brain metastases.

The role of 18F-FACBC PET in the field of neuro-oncology remains to be thoroughly explored. This case report includes the analysis of four sequential 18F-FACBC PET/MRI examinations and investigates their utility in evaluating functional and structural brain metastasis properties.

Case presentation

A middle-aged woman was treated for locally advanced breast cancer (invasive ductal carcinoma, ER+, PgR−, HER2) in 2012. Treatment included neoadjuvant chemotherapy, surgery, adjuvant radiation and hormonal therapy—with curative intent. After 2 more years, she was diagnosed with extracranial metastases and a solitary brain metastasis in the left frontal lobe. The brain metastasis was removed with gross total resection, followed by 18 weekly cycles of chemotherapy. Almost 3 years later, she presented with an 11-mm metastasis in the lateral precentral gyrus of the right brain hemisphere and a 30-mm metastasis in the cerebellum. The cerebellar metastasis was removed with gross total resection and the cerebral metastasis was treated with stereotactic radiotherapy (9 Gy × 3). As part of this study, four 18F-FACBC PET/MRI scans were conducted approximately 1 month apart on a simultaneous PET/MRI scanner (Siemens Biograph mMR, Siemens Healthcare, Erlangen, Germany). Scans 1 and 2 were conducted prior to and 1 month after stereotactic treatment of the cerebral metastasis, respectively. Scan 3 was conducted upon suspicion of a recurrence and showed a 9-mm recurrent cerebellar metastasis that was treated with stereotactic radiotherapy (9 Gy × 3). Scan 4 was conducted approximately 1 month after stereotactic radiotherapy treatment of the cerebellar metastasis (Table 1).

Table 1 Relevant patient medical history and interventions prior to and after PET/MRI acquisitions

MRI sequences acquired included contrast-enhanced 3D T1, 3D FLAIR and ultrashort echo time for attenuation correction. For each of the four scans, the patient was required to fast for a minimum of 4 h prior to the injection of 189.7±8.0 MBq (3 MBq/kg) 18F-FACBC. Intravenous tracer injection occurred at t = 0 of the 35-min scan. PET tracer uptake was recorded in list/dynamic mode. PET image reconstruction was performed on the mMR with iterative reconstruction (3D OSEM algorithm, 3 iterations, 21 subsets, 344 matrix, 4-mm Gaussian filter) with point spread function, decay and scatter correction. MR-based attenuation correction was performed with a deep learning-based method (DeepUTE) using the ultrashort echo time MR sequence as input for making modified MR-based AC maps (Ladefoged et al. 2020).

Static PET analysis

Data analysis was conducted on both lesions using PMOD software (version 4.104, PMOD Technologies LLC, Zürich, Switzerland) and was identical for data acquired from the four scans. Standard uptake values (SUVs) were evaluated using the patient’s body weight (SUVbw) from static PET images. Spherical volumes of interest (VOIs) were placed to encompass the lesion to calculate tumour SUVmax and SUVpeak. A 2-mL VOI was placed on the contralateral side of the brain to calculate background SUV (SUVbkg). Using Eq. 1, peak and max tumour-to-background ratios (TBRs) were calculated for static PET images. TBRpeak was calculated for the cerebral tumour. Due to its small volume, only TBRmax was calculated for the cerebellar tumour.

$$ {\displaystyle \begin{array}{l}\left({TBR}_x={SUV}_{x\;(tumour)}/{SUV}_{mean\;(background)}\right)\\ {}x=\mathit{\max}\ or\ peak\end{array}} $$

All four PET scans revealed a metastasis in the caudal part of the precentral gyrus of the right brain hemisphere (Fig. 1), while the latter two scans revealed a metastasis caudomedially in the left cerebellar hemisphere (Fig. 2). All scans exhibited low background 18F-FACBC uptake and high tumour 18F-FACBC uptake, with significantly higher uptake in the cerebral tumour compared to the cerebellar tumour (Tables 2 and 3). Cerebral tumour TBRpeak decreased between the first two scans following stereotactic radiotherapy and increased back to the initial values for scans 3 and 4. TBRpeak was the largest in scan 1. Cerebellar tumour TBRmax decreased between scans 3 and 4 (Table 3).

Fig. 1
figure 1

Static PET, T1 MRICE and fused PET/MR images of the cerebral tumour from scans 1–4. Axial view of the patient’s metastasis in the lateral precentral gyrus of the right hemisphere acquired using hybrid PET/MR imaging at four time points: scan 1—before stereotactic radiation, scan 2—1 month after the end of stereotactic radiation, scan 3—on suspicion of recurrence 1 month after scan 2, and scan 4—1 month after scan 3. Static PET (20–35 min), T1 MRICE and fused PET/MR images are shown from the top to bottom rows, respectively. Columns ad show scans 1–4. The SUV scale for static PET images was 0–3.3, and the SUV scale of fused PET/MR images was 0.45–3.3

Fig. 2
figure 2

Static PET, T1 MRICE and fused PET/MR images of the cerebellar tumour in scans 3 and 4. Axial view of the patient’s left hemisphere caudomedial cerebellar metastasis (arrow) acquired using hybrid PET/MR imaging at time points 3 and 4. Static PET (20–35 min), T1 MRICE and fused PET/MR images are shown from the top to bottom rows, respectively. Columns a and b show scans 3 and 4. The SUV scale for static cerebellar PET images was 0–2, and the SUV scale of fused PET/MR images was 0.45–2

Table 2 Summary of static PET and volumetric PET/MRI cerebral tumour data for all four scans
Table 3 Summary of static PET and volumetric PET/MRI cerebellar tumour data for scans 3 and 4

Throughout scans 2–4, it was difficult to distinguish viable tumour tissue from tissue reactions to radiotherapy in both metastases with clinical MRI. An MRI 1 month after scan 4 showed an increase in lesion volume, the patient was treated with a gross total resection of the cerebral tumour and histological findings confirmed tumour progression.

Dynamic PET analysis

The tumour VOIs generated for static PET analysis were also used to calculate TBRpeak for dynamic data. Due to the smaller size of the cerebellar lesion, dynamic PET analysis was only conducted on the cerebral lesion. Dynamic PET data for all four scans showed that TBRpeak in the cerebral tumour peaked within the first 2 min after injection, at 65, 52.5, 105, and 115 s, respectively (Fig. 3). TBRpeak reached a steady-state after 10 min and was sustained for the remainder of the 35-min scan.

Fig. 3
figure 3

Graph showing cerebral tumour TBRpeak as a function of time for all four scans. TBRpeak values for each of the four scans peak within the first 2 min, followed by a decrease and a steady-state after 10 min

Tumour volumes

For the PET and MRI tumour volume calculations, static PET (20–35 min) images were registered to T1 MRICE images in PMOD in order to get a perfect alignment between the two image modalities. PET tumour volumes were calculated by generating three-dimensional iso-contours inside a spherical VOI placed to encircle the entire tumour PET tracer uptake region. Iso-contour boundaries were defined as the region with PET tracer uptake > 2 × SUVbkg, and regions of high non-tumour PET tracer uptake were manually erased. T1 MRICE tumour volumes were determined by manually tracing tumour boundaries for each axial slice. Intersecting PET and MRI tumour volumes were calculated in PMOD using PET and MRI tumour volume VOIs. For all time points, the PET volumes were larger than, and enclosed, T1 MRICE volumes (Tables 2 and 3) for the cerebral tumour (see example, Fig. 4). Both PET- and MRI-based cerebral tumour volumes decreased between scans 1 and 2, followed by a slight increase in scan 3 and then an increase to almost 2× initial values in scan 4 (Table 2). Cerebral TBRpeak values correlated positively with PET and MRI volume changes for the first two scans (Table 2) but returned to initial values in scans 3 and 4. Relative PET and MRI volume changes were near identical and followed the same trend throughout the four scans.

Fig. 4
figure 4

PET and MRI VOIs of cerebral tumour from scan 4. An example of the relationship between PET (green) and MRI (blue) tumour volumes that occurred in all four scans. a Zoomed in axial view of T1 MRICE image and b PET image from scan 4. c 3D representation showing PET VOI encompassing MRICE VOI from scan 4

Both PET- and MRI-based cerebellar tumour volumes decreased between scans 3 and 4 (Table 3), and this coincided with a decrease in tumour TBRmax following stereotactic radiotherapy. However, the PET volume showed a relatively larger decrease than the MRI volume, resulting in a slightly smaller PET than MRI volume in scan 4.


This is the first study aimed to evaluate 18F-FACBC performance of brain metastases using four consecutive PET/MRI examinations. The results of this study show that 18F-FACBC could provide useful information with regard to tumour detection. In particular, there was low 18F-FACBC tracer uptake (SUVbkg) in healthy brain tissue as well as high tracer uptake (SUVpeak and SUVmax) (Tables 2 and 3) in both cerebral and cerebellar tumour VOIs, consistent with previous studies on gliomas (Bogsrud et al. 2019; Karlberg et al. 2017; Karlberg et al. 2019; Tsuyuguchi et al. 2017; Michaud et al. 2020). We reported an increase in 18F-FACBC uptake in healthy brain tissue (SUVbkg) after radiation therapy in scan 2 (Table 2), but regardless of this increase, the distinctively low 18F-FACBC tracer uptake in healthy tissue (SUVbkg) played a large role in the high tumour contrast (TBRmax and TBRpeak) in this patient. Previous studies using AA tracers 11C-MET and 18F-FET on brain metastases reported higher SUVbkg and on average lower TBRmax values than 18F-FACBC in the current study (Gempt et al. 2015; Grosu et al. 2011; Ceccon et al. 2017), probably caused by differences in the transport system used by the various AA tracers. 11C-MET and 18F-FET rely primarily on the system L amino acid transporter (LAT1) for accumulation in cancerous cells (Sun et al. 2018; Heiss et al. 1999; Habermeier et al. 2015; Ono et al. 2013), while 18F-FACBC utilises both LAT1 and the alanine-serine-cysteine transporter 2 (ASCT2) (Ono et al. 2013; Okudaira et al. 2015). Since 18F-FACBC has a higher affinity for ASCT2 than LAT1, and since ASCT2 is not expressed on the luminal side of the blood vessels, this could potentially lead to a lower transport of 18F-FACBC compared to 18F-FET and 11C-MET across the intact BBB in healthy tissue. In line with these results, Michaud et al. (Michaud et al. 2020) have indeed demonstrated that the transport across the intact BBB in healthy tissue in patients with recurrent gliomas was around 6 times higher for MET compared to 18F-FACBC. In tumour tissue with a broken BBB, the transport will be increased for all the AA-tracers. But since 18F-FACBC mainly relies on ASCT2 transport, which is expressed on the abluminal side of the blood vessels, this could further increase the uptake for 18F-FACBC compared to 18F-FET and 11C-MET in tumour tissue with a broken BBB, further increasing the TBR seen for 18F-FACBC. It has been shown that ASCT2 is likely more active in early stages of tumour development, whereas LAT1 predominates with increasing acidity from the cell density that increases with tumour progression (Oka et al. 2012). This could indicate that 18F-FACBC is a better AA PET tracer in detecting early stages of tumour development, while 11C-MET and 18F-FET uptake correlates more with advanced tumour progression and recurrence than 18F-FACBC. Further studies should be conducted to elucidate 18F-FACBC pharmacokinetics and how it is affected by pathological cellular changes, as well as evaluate the promising contrast provided by 18F-FACBC in brain metastases.

Notably, the cerebellar tumour was detected by 18F-FACBC PET in scans 3 and 4, despite having a volume of less than 0.2 ccm in scan 4 (Table 3). This is particularly noteworthy as it supports a previous study which reported that the high TBR provided by 18F-FACBC PET allowed the detection of small satellite tumours that were not registered by MRI (Bogsrud et al. 2019). This is a promising result with regard to early detection of brain metastases that might otherwise be overlooked.

Dynamic evaluation of the cerebral tumour TBRpeak showed a time-to-peak (TTP) of less than 2 min for all scans, followed by a decrease reaching a steady-state within 10 min which persisted for the remainder of the examination. This steady-state provided an ideal time frame for static PET analysis, justifying the 20–35-min time frame used in this study, and is comparable to the 10–20-min time frame recommended by guidelines for static AA PET analysis using 11C-MET and 18F-FET (Law et al. 2019). These findings are consistent with 18F-FACBC uptake in high-grade gliomas reported by Karlberg et al. and Kondo et al. which described a steady-state following a TTP of 43 s and < 10 min, respectively (Karlberg et al. 2019; Kondo et al. 2016). The similarity between brain metastasis and glioma AA PET uptake patterns should be further explored to better understand tumour physiology and AA PET tracer uptake.

Studies have suggested that dynamic 18F-FET uptake can be used for tumour grading, where a slow increase in the dynamic uptake in low-grade gliomas and a short TTP followed by a decrease in the high-grade gliomas has been demonstrated (Pöpperl et al. 2007). The dynamic 18F-FET PET analysis in patients with brain metastases has on the other hand demonstrated a broad distribution of TTP and time-activity curves throughout metastases of different primary tumours (Unterrainer et al. 2017). In this study investigating brain metastases from breast cancer, TTP and time-activity curves using 18F-FACBC showed the same pattern across all four scans regardless of radiotherapy, but no conclusions with regard to its diagnostic value can be reached based on the paucity of data of 18F-FACBC uptake analysis in metastases.

When investigating pathology in the brain, inadequate transport across the intact BBB can lead to limited diagnostic performance and the production of erroneous results, invalidating the usefulness of the tracer as a supplement to contrast-enhanced MRI. MR contrast agents do not cross the intact BBB, and as already noted, the transport across the intact BBB is shown to be extremely low for FACBC (Michaud et al. 2020). Nevertheless, 18F-FACBC uptake has been observed in some progressive and recurrent gliomas that were not defined with contrast-enhanced MRI (Bogsrud et al. 2019; Michaud et al. 2020), and it has also been shown that 18F-FET PET tumour margins differed significantly from those calculated from MRI in patients with brain metastases (Gempt et al. 2015). This suggests that CE and AA tracer uptake varies when it comes to transport across the intact and broken BBB and that combined PET/MRI data may help describe more exact brain metastasis margins. In this study, tumour boundaries were defined by regions with PET tracer uptake >2 × SUVbkg. When using this threshold, PET volumes were consistently larger than and enclosed the vast majority of T1 MRICE volumes. However, compared to 18F-FET-examinations, where the thresholds for defining FET-based volumes have been verified with histological analysis (Pauleit et al. 2005; Law et al. 2019), there are no established guidelines for which threshold that should be used for 18F-FACBC PET volumetric brain tumour analysis. Because of this, the usefulness of 18F-FACBC PET/MRI in the volumetric tumour analysis of brain metastases cannot yet be determined.

In this study, the small cerebellar metastasis was discovered simultaneously in scan 3 with PET and MRI. Due to a lack of studies, it was uncertain whether 18F-FACBC uptake would correspond to radiotherapy-induced pseudo-progression or actual tumour progression—as has been shown with 11C-MET and 18F-FET (Grosu et al. 2011; Akhoundova et al. 2020). In this case, however, the latter was found to be true; gross total resection and histology of the cerebral tumour confirmed tumour progression—a distinction not possible to make with MRI alone.

There are clear limitations to this study, such as the limited sample size of only one patient and its low reproducibility due to a lower availability of PET/MRI than PET/CT. There are also limitations in the MR-based method used for attenuation correction purpose in postoperative patients with craniotomies and metal implants, and in children, since the method for the updated software is only evaluated in adults (Ladefoged et al. 2020). However, the patient in the current study did not have any implants in the brain that could interfere with the MR-based attenuation correction method, and this issue should therefore not affect the results. Also, there are limitations for calculating 18F-FACBC-based tumour volumes, since no established thresholds exist for this tracer in brain tumours yet. More studies should be performed to correlate 18F-FACBC uptake with histological findings to determine appropriate thresholds, and to further investigate whether 18F-FACBC can help delineate tumour spread more accurately compared to MRI only.


This study describes a potentially promising use for 18F-FACBC PET in brain metastases. 18F-FACBC provided high TBRpeak values in brain metastases, mostly due to a consistently low tracer uptake in healthy tissue (SUVbkg). This shows that 18F-FACBC may be used as a tool for visualising brain metastases effectively. There was even high 18F-FACBC uptake in the small cerebellar metastasis, which suggests that 18F-FACBC could play an important role in detecting brain metastases that might otherwise be overlooked. However, more studies are needed to correlate 18F-FACBC PET uptake with histological findings to determine appropriate thresholds for 18F-FACBC-based brain tumour volume definition.

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.



Anti-1-amino-3-[18F]-fluorocyclobutane-1-carboxylic acid


Positron emission tomography


Magnetic resonance imaging


Amino acid










Tumour-to-background ratio


Fluid-attenuated inversion recovery


Standard uptake value


Volume of interest


System L amino acid transporter 1


Alanine-serine-cysteine transporter 2


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The authors would like to thank Katy-Olga Grøtte Stene at the Department of Oncology for recruiting the patient and the staff at the PET-centre for their cooperation with the PET/MRI examinations. We would also like to thank the Trond Mohn Foundation for supporting us with funding for the project.


This work was supported by the Trond Mohn Foundation.

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Authors and Affiliations



KJ: formal analysis, writing of the original draft, and visualisation. EMB: validation, writing, review, and editing. HJ: validation. TSS: validation, resources, writing, review, and editing. AK: conceptualization; methodology; investigation; writing, review, and editing; and supervision. LE: conceptualization; methodology; investigation; writing, review, and editing; supervision; and project administration. The author(s) read and approved the final manuscript.

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Correspondence to Live Eikenes.

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Ethics approval and consent to participate

The patient gave informed consent to participate in an ongoing study evaluating the additional value of 18F-FACBC PET with simultaneous MRI before and after stereotactic radiotherapy. The study was approved by the Regional Ethical Committee (REK, reference number 2018/2243).

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Written informed consent was obtained from the patient for publication of this case report and accompanying images.

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The authors declare that they have no competing interests.

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Johannessen, K., Berntsen, E.M., Johansen, H. et al. 18F-FACBC PET/MRI in the evaluation of human brain metastases: a case report. European J Hybrid Imaging 5, 7 (2021).

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