Skip to main content

Lymphopenia during 177Lu-DOTATATE therapy leading to recurrence of tuberculosis: a case report


We report the case of a 72-year-old woman who presented with tuberculous arthritis during the setting of 177Lu-DOTATATE therapy for a grade-2 neuro-endocrine pancreatic tumor with liver metastases. We hypothesized that this recurrence might have been related to the occurrence of lymphopenia, which is common during PRRT. Indeed, though lymphopenia is frequently dismissed, it could lead to the development of opportunistic diseases and its severity should be examined, especially in case of abnormal clinical symptoms.


Peptide receptor radionuclide therapy (PRRT) in NET has shown good results in metastatic patients with grade-1 or grade-2 tumor originating from the midgut (Oronsky et al. 2017; Mortenson and Bold 2002; Anderson and Bennett 2016). PRRT targeting somatostatin receptor especially SSTR2 demonstrated increased objective response rate, overall survival and quality of life (Bodei et al. 2015). In NET patients with primary pancreatic tumors, PRRT showed promising results in non-randomized studies, and though phase III results are pending (NCT02489604), it is often recommended as a second-line treatment with rare adverse events (Strosberg et al. 2015, 2018). Lymphopenia is frequently reported during PRRT, with transient lymphopenia reported in 18% of patients in the NETTER-1 trial without further impact on the course of PRRT (Marinova et al. 2018; Strosberg et al. 2017). Yet, the occurrence of unusual clinical symptoms in PRRT patients presenting with acute or severe lymphopenia should not be systematically dismissed as insignificant, as it might be lead to an underlying infectious disease.

Material and methods

We reported the case of a 72-year-old woman for whom a history of primary tuberculosis infection during childhood was unclear and who presented with tuberculous arthritis during the setting of 177Lu-DOTATATE PRRT. Initially, this patient had abdominal pain at diagnosis and a contrast-enhanced computer tomography (CE-CT) lead to the diagnosis of primary pancreatic NET with synchronous liver metastases (grade 2 and Ki-67 15% at biopsy). The patient received a first-line treatment using somatostatin analogues without significant tumor response or improvement of the clinical symptomatology. A second and third line of treatment using chemotherapy (gemcitabine/oxaliplatin followed by capecitabine/temozolomide) only stabilized the disease for less than a year. Indeed, a follow-up FDG PET/CT scan showed a mild metabolic progression in both primary pancreatic tumor and liver metastases. 68 Ga-DOTATATE PET/CT showed high uptake in both primary pancreatic tumor and liver metastases (Additional file 1: Fig. S1 ). Therefore, in agreement with the standard of care and after approval of the dedicated multidisciplinary board, initiation of PRRT using 177Lu-DOTATATE was decided. Patients or the public were not involved in the design, or conduct, or reporting, or dissemination plans of this case report.

PRRT Therapy

The patient received the first cycle (C1) of PRRT with a reduced activity of 5.6 GBq instead of standard 7.4 GBq due to her low body mass index (18 kg/m2) though clinical examination was unremarkable and the blood work was within normal range (Fig. 1). Between the first and second cycle, the patient reported a traumatic fall involving the right elbow and the right ankle with persistent inflammatory pain in the latter. At clinical examination before the second treatment, there was a limping of the right ankle with pain at mobilization and swelling, but no redness nor fever was found. In light of those symptoms, PRRT was postponed until further investigations. Same day blood work showed elevated white cell counts with 13.5 (N > 1.8) G/L for neutrophils (NP), whereas lymphocytes (LP) were below 0.4 (N > 1.5) G/L (Fig. 1). In addition, C-reactive protein (CRP) was elevated at 102 (N < 5) mg/L, but procalcitonin was normal at 0.08 (N < 0.5) µg/L) and blood cultures were negative. Similarly, synovial fluid withdrawal did not show any inflammatory nor cancerous cells nor crystals, and standard ankle and chest X-rays were also negative (Additional file 1: Fig. S2). Thus, the resulting diagnostic was a mono-arthritis of undefined origin ranging from paraneoplastic, chondrocalcinosis, inflammatory rheumatism to insidious infection. Additionally, as the blood work normalized the next day without any treatment (NP: 4.9 G/L and LP: 0.65 G/L, Fig. 1) and in the absence of conclusive clinical sign of active infection, the patient received the second cycle of PRRT with a further reduced activity of 3.8 GBq of 177Lu-DOTATATE with good clinical tolerance. Clinical assessment before injection of the third injection of 177Lu-DOTATATE was normal, and the patient did not present any pain at the clinical examination of her right ankle. Between the 3rd and 4th cycle of PRRT, the patient was again investigated for the same pain of the right ankle, which was explored without conclusive results leading to a delay in initiating the 4th injection of 177Lu-DOTATATE by 4 weeks. At the time of C4,  the blood work was satisfactory though a slight Lymphopenia was seen 1.4 G/l (Fig. 1), and the treatment was carried out with injection of  half the standard activity  of 177Lu-DOTATATE with a good clinical tolerance.

Fig. 1
figure 1

Changes in white blood cell counts during PRRT (lymphocytes (blue) and neutrophils (orange); logarithmic scale); dotted line: definition of severe lymphopenia (value < 0.5G/l); * in red: values for CRP (mg/ml)

Interestingly, though all investigations remained negative during the course of PRRT, post-injection planar scintigraphy at 48 h showed from the second cycle an increasing mild uptake of the right angle (Fig. 2). In contrast, on additional SPECT/CT imaging, there was a continued decrease in Lu177-DOTATATE uptake in NET lesions, particularly in the pancreatic tumor and liver metastases (Fig. 3). Ultimately, the patient presented with a good response on the 68 Ga-DOTATATE PET/CT scan done 4 months after the end of PRRT (Additional file 1: Fig. S1).

Fig. 2
figure 2

Whole body planar scintigraphy images in the anterior view 2-days post PRRT with zoom images below showing increasing uptake in the right ankle from cycle 2 to 4

Fig. 3
figure 3

Axial slices of SPECT, CT and fused SPECT/CT images showing uptake in the primary pancreatic tumor and liver metastases after each cycle of PRRT

However, two months after the end of PRRT, the patient experienced once again an acute pain in the right ankle. Blood tests, ankle ultrasound and CT scan were suspicious for an active infection, with the latter showing bone erosions in favor of an aggressive process. The patient underwent an arthroscopy with multiple biopsies, an arthrotomy of the medial malleolus and a curettage of the infection site. Biopsies and subsequent bacterial cultures led to retain the diagnosis of a chronic tuberculosis infection with mycobacteria tuberculosis of the right ankle. As tuberculosis was suspected, a thoracic CT scan was performed and did not show any pulmonary sign of the disease. After a pneumology referral, a long-term anti-tuberculosis treatment using the four-drug fixed-dose combination regimen RIMSTAR® (9), combined with a B6 vitamin substitution, was started.


To the best of our knowledge, this is the first study reporting the likely recurrence of mycobacteria tuberculosis during the course of PRRT in patients with NET. This finding is interesting because adverse events or abnormal non-tumoral uptakes of 177Lu –DOTATATE are uncommon (Strosberg et al. 2015, 2018). Indeed, lymphopenia was frequent and has been reported in 18% of the patients in NETTER-1 trial but it was usually transient and of no consequence during the course of PRRT (Strosberg et al. 2017). Most common adverse events in the NETTER-1 trial were gastro-intestinal disorders, fatigue, musculoskeletal pain followed by blood disorders mainly thrombocytopenia (25%), lymphopenia (18%) and anemia (14%), which were mainly moderate (Strosberg et al. 2017). Thus, despite post-injection planar scintigraphy showing an increasing uptake of the right ankle for this patient from C2 to C4, this uptake was considered to be a false-positive related to local inflammation (Fig. 2). Especially, since all SPECT/CT images done after cycle 2 to 4, all showed a decrease in NET lesions uptake suggestive of an early response to PRRT which was not coherent with the appearance of new secondary bone locations (Fig. 3). Additionally, the patient had a history of traumatic fall between C1 and C2 and all investigations remained negative for an active infection. Indeed, it has been reported in the literature that inflammatory cells such as lymphocytes and macrophages also expressed SSTR2, which was in our opinion coherent with a reactive process. Nonetheless, the patient did present with an acute and severe lymphopenia during the setting of PRRT, which is often underestimated even so it showed the highest level of grade 3 and 4 events (9%) in the NETTER-1 trial (8, 10). Indeed, its presence does not currently require the nuclear physician to postpone or reduce the activity of 177Lu-DOTATATE to be injected. However, it has been demonstrated in the literature that acute and severe lymphopenia could lead to the recurrence or occurrence of opportunistic disease in immunosuppressed patients mainly transplants or HIV patients but also impact outcomes in patients with sepsis especially with LP < 0.5G/L (Fuehner et al. 2019). Thus, when we looked retrospectively at the patient history that is the concomitant right ankle pain with an episode of severe lymphopenia and the abnormal uptake on the post-treatment planar scintigraphy, we hypothesized that it could have been associated with the recurrence of this tubercular disease. Besides, mycobacteria tuberculosis is not a rare pathogen and even so patients could not present with a clear history of primary infection and it should be considered in front of abnormal or unexplained clinical symptoms associated with lymphopenia (Fuehner et al. 2019; Kaul and Chauhan 2014; Sreejith et al. 2010; Cock et al. 1995). Interestingly, except for the blood work done prior to PRRT initiation and the one before the last cycle (Fig. 1), the patient always presented with at least moderate lymphopenia raising the question of systematic screening for latent tuberculosis in at-risk patients before PRRT initiation (Vries et al. 2014). This might be particularly interesting in elderly patients with an inconclusive history or those in a precarious situation especially for patients with borderline LP counts at PRRT initiation and/or were previously treated with potentially hematotoxic treatments. Indeed, post-treatment lymphopenia has been reported in various solid tumors in patients treated with chemotherapy or radiation therapy (Wang et al. 2021; Campian et al. 2013). In this case, the patient was previously treated with 2 lines of chemotherapy and there was a tendency to mild lymphopenia with a lymphocyte cells count of 1.2 G/l seen 2 months before the start of PRRT while neutrophils, hemoglobinemia and platelets were all within normal range. The presence of local inflammation following the traumatic fall reported by the patient may have been an additional relevant factor leading to the recurrence of tuberculosis at this particular site.

Thus, our report showed that lymphopenia might not be an insignificant blood finding and could require further investigations, especially in front of abnormal clinical symptoms during the course of PRRT. This is all the more important that severe lymphopenia could occur at reduced dose as for this patient, who received a dose lower than the current fixed dose of 7.4 GBq recommended in the literature (Bodei et al. 2015), (Strosberg et al. 2015, 2018)


To resume, though lymphopenia is frequent and usually benign during the course of PRRT it could be associated with opportunistic diseases and its severity should be examined especially in case of abnormal clinical symptoms.

Availability of data and material

Data and material can be made available upon request.



Neuro-endocrine tumor


Peptide receptor radionuclide therapy


Somatostatin receptor 2


Contrast-enhanced computer tomography


C reactive protein








  • Anderson CW, Bennett JJ (2016) Clinical presentation and diagnosis of pancreatic neuroendocrine tumors. Surg Oncol Clin N Am 25:363–374

    Article  Google Scholar 

  • Bartacek A, Schutt D, Panosch B et al (2009) Comparison of a four-drug fixed-dose combination regimen with a single tablet regimen in smear-positive pulmonary tuberculosis. Int J Tuberc Lung Dis 13:760–766

    CAS  Google Scholar 

  • Bodei L, Kidd M, Paganelli G et al (2015) Long-term tolerability of PRRT in 807 patients with neuroendocrine tumours: the value and limitations of clinical factors. Eur J Nucl Med Mol Imaging 42:5–19

    Article  CAS  Google Scholar 

  • Campian JL, Ye X, Brock M et al (2013) Treatment-related lymphopenia in patients with stage III non-small-cell lung cancer. Cancer Invest 31(3):183–188

    Article  Google Scholar 

  • De Cock KM, Grant A, Porter JD (1995) Preventive therapy for tuberculosis in HIV-infected persons: international recommendations, research, and practice. Lancet 345:833–836

    Article  Google Scholar 

  • de Vries G, Aldridge RW, Cayla JA et al (2014) Epidemiology of tuberculosis in big cities of the European Union and European Economic area countries. Euro Surveill 19(9):20746

    Article  Google Scholar 

  • Elliott DE, Li J, Blum AM et al (1999) SSTR2A is the dominant somatostatin receptor subtype expressed by inflammatory cells, is widely expressed and directly regulates T cell IFN-Œ≥ release. Eur J Immunol 29:2454–2463

    Article  CAS  Google Scholar 

  • Fuehner T, Ernst D, Greer M et al (2019) Opportunistic infections in the immunocompromised host. The European Respiratory Society, ERS Handbook Respiratory Medicine, pp 400–410

    Google Scholar 

  • Kaul A, Chauhan TS (2014) Opportunistic infection in renal transplant recipients. Indian J Transpl 8:S57–S64

    Article  Google Scholar 

  • Marinova M, Mücke M, Mahlberg L et al (2018) Improving quality of life in patients with pancreatic neuroendocrine tumor following peptide receptor radionuclide therapy assessed by EORTC QLQ-C30. Eur J Nucl Med Mol Imaging 45(1):38–46

    Article  CAS  Google Scholar 

  • Mortenson M, Bold RJ (2002) Symptomatic pancreatic polypeptide-secreting tumor of the distal pancreas (PPoma). Int J Gastrointest Cancer 32:153–156

    Article  Google Scholar 

  • Oronsky B, Ma PC, Morgensztern D et al (2017) Nothing but NET: a review of neuroendocrine tumors and carcinomas. Neoplasia 19:991–1002

    Article  CAS  Google Scholar 

  • Sreejith P, Jha V, Kohli HS et al (2010) Allograft and prostatic involvement in a renal transplant recipient with disseminated tuberculosis. Indian J Nephrol 20:40–42

    Article  CAS  Google Scholar 

  • Strosberg J, Wolin E, Chasen B et al (2015) 6LBA 177-Lu-Dotatate significantly improves progression-free survival in patients with midgut neuroendocrine tumours: results of the phase III NETTER-1 trial. Eur J Cancer 51:S710

    Article  Google Scholar 

  • Strosberg J, El-Haddad G, Wolin E, Hendifar A, Yao J, Chasen B, Mittra E, Kunz PL, Kulke MH, Jacene H, Bushnell D (2017) Phase 3 trial of 177Lu-Dotatate for midgut neuroendocrine tumors. N Engl J Med 376(2):125–135

    Article  CAS  Google Scholar 

  • Strosberg J, Wolin E, Chasen B et al (2018) Health-related quality of life in patients with progressive midgut neuroendocrine tumors treated with (177)Lu-dotatate in the phase III NETTER-1 trial. J Clin Oncol 36:2578–2584

    Article  CAS  Google Scholar 

  • Wang JL, Ma R, Kong W et al (2021) Lymphopenia in esophageal cancer: what have we learned? Front Oncol 11:625963

    Article  Google Scholar 

Download references


To the patient for agreeing to participate to this case report.


No funding was received for this study.

Author information

Authors and Affiliations



The first author (S.B) contributed to the design of the study, data collection, data analysis and writing of the manuscript. The last author (N.S), M.D, M.M.C, M.F and J.O.P contributed to editing of the manuscript. All authors approved the final version of this manuscript.

Corresponding author

Correspondence to Sarah Boughdad.

Ethics declarations

Ethical approval and consent to participate

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Conflict of interest

The authors declare no conflict of interest related to this study.

Informed consent

Informed consent was obtained from the participant included in the study.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Additional file 1:

Fig. S1. 68Ga-DOTATATE PET/CT image (SUV scale: 0–12.5 g/mL): MIP images a and e before PRRT and after PRRT; b), c) and d) axial slices CT, Fused PET/CT and CE-CT (Top images=after PRRT). Fig. S2. Chest (a) and right ankle X-rays (front (b) and side (c)) before second injection of 177Lu-DOTATATE.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Boughdad, S., Da Mota, M., Mendes De Carvalho, M. et al. Lymphopenia during 177Lu-DOTATATE therapy leading to recurrence of tuberculosis: a case report. European J Hybrid Imaging 6, 36 (2022).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: